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"improvement": "", "progress": "", "follow_up": "", "order_no": ""}], "negatives": [{"sales": "叶信福", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "杨梦华课题组", "date": "2026.7.2", "competitor": "", "direction": "负", "feedback": "客户反馈我们的蛋白胶存在边界不太清晰，插入梳子后比较歪", "detail": "", "solution": "与之前情况一并反馈", "tech": "", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "叶信福", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "马瑞杰课题组", "date": "2026.6.30", "competitor": "", "direction": "负", "feedback": "客户反馈我们的蛋白胶存在边界不太清晰的问题", "detail": "客户加的不算太快，但是还是会出现一定的分界不清晰", "solution": "与之前情况一并反馈", "tech": "", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "王晓杰课题组", "date": "2026.6.29", "competitor": "新赛美快速制胶", "direction": "负", "feedback": "我们的蛋白胶需要缓慢加才能相对平整，新赛美的不需要", "detail": "在用新赛美一步法制胶，之前是用的我们的一步法制胶，上下层出现分界不明显，新赛美的倒进去也不影响分层", "solution": "需改进", "tech": "", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR4D441、BR4D421", "category": "快速转膜液、快速封闭液", "group": "邹鹏课题组", "date": "2026.6.29", "competitor": "", "direction": "负", "feedback": "用我们的转膜液然后用碧云天的封闭液出现大于70的蛋白黑膜，小于70的正常。100多的转膜没完全转过去，转了30分钟。右边是平时做的，新赛美的转了40分钟", "detail": "", "solution": "需研发验证", "tech": "", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260608050"}, {"sales": "袁殿果", "product": "BR1A401", "category": "快速PCR酶", "group": "黄志峰课题组", "date": "2026.6.29", "competitor": "", "direction": "负", "feedback": "客户用快p做小鼠鉴定，但是是用的降落PCR跑的，退火53度，条带500bp左右，延伸时间35s，有淡淡的非特异性条带", "detail": "", "solution": "建议客户用三部法程序来跑，退火可以55-58度。且延伸时间可以短一点，5s就足够。", "tech": "", "is_product_issue": "", "is_resolved": "跟进后续测试结果", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260610005"}, {"sales": "叶信福", "product": "BR3M121", "category": "高保真酶", "group": "黄家保课题组", "date": "2026.6.29", "competitor": "", "direction": "负", "feedback": "客户反馈二轮扩增无条带或者有非特异性扩增", "detail": "客户使用第一次PCR产物当模板，20ul体系，模板加1ul。会出现第二次扩增无条带，或者出现突变的情况。", "solution": "建议客户把第一次PCR产物稀释20倍后再进行第二次扩增，同时建议客户第二次的循环数可以减少到25。", "tech": "王凡", "is_product_issue": "", "is_resolved": "跟进后续测试结果", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR1D303", "category": "一步法逆转录", "group": "黄云鹏课题组", "date": "2026.6.23", "competitor": "康为CW3371m", "direction": "负", "feedback": "提取RNA后测浓度没问题，用我们的一步法逆转录之后用诺唯赞Q712做qpcr没有跑出来", "detail": "客户最开始用我们优化前的BR1D303逆转录，搭配诺唯赞Q712没有结果数据异常，目前还没拿到客户实验实验数据。后客户反馈用优化后的BR1D303测试还是没有结果。样本是果蝇提取的RNA。具体信息需线下收集。", "solution": "周五下午线下了解客户具体实验细节情况，有可能的话现场测试。", "tech": "", "is_product_issue": "", "is_resolved": "第一位客户使用的那一批试剂是老款，导致的性能上有问题。第二位客户测试时，融解曲线程序不小心删除导致没有融解曲线是正常现象，也不影响 CT值。但是出现扩增曲线平台期低的问题。 经过比对发现，扩增曲线中如图出现了几个异常数据值导致起始的荧光值就很高，到达将近10000（如图），但是正常的峰值应该是在8左右，所以仪器在自动识别阈值时出现了问题，导致扩增曲线看起了比较低。但是对比和康为的峰值，其实是一致的。", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1A401", "category": "快速PCR酶", "group": "张俊红课题组", "date": "2026.6.19", "competitor": "", "direction": "负", "feedback": "客户反馈我们BR1A401扩增特异性不好，有非特异性条带。", "detail": "客户做的是菌液PCR，反馈有杂带，引物推荐退火是58度，但是客户用53度跑，出现多条带，并且坚定认为是产品问题。", "solution": "今天线下测试，提高退火60度做PCR没有问题。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "林咸明课题组", "date": "2026.6.17", "competitor": "", "direction": "负", "feedback": "客户反馈蛋白胶在小分子和大分子条带转膜效果不好，但是蛋白Maker是没有问题的", "detail": "客户使用12.5%的蛋白胶，跑全蛋白转膜后用丽春红染色后，小分子蛋白和大分子没有明显条带。但是蛋白Maker是没有问题的，客户用的自己配制的转膜液，转膜时间客户需要加长。", "solution": "需研发验证我们的蛋白胶和在客户的转膜液下的转膜效果。客户也会搭配我们的转膜液再次测试", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR3M121", "category": "高保真酶", "group": "陶曾课题组", "date": "2026.6.16", "competitor": "", "direction": "负", "feedback": "客户反馈高保真酶扩增有拖带现象", "detail": "客户扩增片段5000bp左右，延伸时间是50s，胶图有少量拖带，建议客户减少循环数，同时时间可以减少一点", "solution": "已经建议客户减少循环数，同时时间可以减少一点，同时退火问题也可以摸索一下。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "待测试结果", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1C101", "category": "同源重组酶", "group": "项黎新课题组", "date": "2026.6.15", "competitor": "", "direction": "负", "feedback": "客户反馈同源重组酶，单克隆少，阳性率低。", "detail": "客户之前采购使用没有问题，最近这一批批号20251122使用的效率低，客户是单片段和双片段都有，单克隆数量很少，只有几个，且都是假阳性。了解到客户片段500bp左右，载体6000bp左右，但是载体浓度较低，只有十几ng。", "solution": "已经将客户物料寄回研发测试，测试表面该批次的连接效率确实存在问题，已经给客户换货，客户同意再次测试。", "tech": "王凡", "is_product_issue": "是", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1F103", "category": "一步法qrt", "group": "金崇伟课题组", "date": "2026.6.3", "competitor": "", "direction": "负", "feedback": "跟进5.18测试反馈，qrt后续没在测试", "detail": "客户试剂保存没有问题，在有效期内。了解客户RNA质量，客户表示提取时就不太好，可能样本存在问题。", "solution": "约定后续线下指导客户实验", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR1E102", "category": "qPCR酶", "group": "刘小龙课题组", "date": "2026.6.2", "competitor": "诺唯赞Q331", "direction": "负", "feedback": "用诺唯赞和我们的共做了四组数据其中两组数据一致，两组数据相反", "detail": "逆转录都是用BR1D303，qpcr用诺唯赞Q331和我们的BR1E102做了对照第三和第四组结果趋向一致，但第一、二组恰好相反，我们的CT值确实比诺唯赞小。", "solution": "初步判断客户可能出现加样错误，等待客户后续测试结果", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260512022"}, {"sales": "刘静文", "product": "BR4D441", "category": "快速转膜液", "group": "陆华松课题组", "date": "2026.6.1", "competitor": "自己配转膜液", "direction": "负", "feedback": "30min大蛋白转不干净", "detail": "客户转膜液第一次用正常，但是第二次回收后相同时间会出现大蛋白转膜不彻底的问题。", "solution": "建议客户使用回收的转膜液时可以少量补充无水乙醇，同时转膜时间可以适当加长5-10min。不建议多次回收使用。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "尤争英课题组", "date": "2026.5.25", "competitor": "雅酶两部法制胶试剂盒", "direction": "负", "feedback": "", "detail": "客户测试一步法制胶，上下层分界线模糊，建议客户加上层胶的时候要缓慢，太快会把下层胶冲散，导致分界线模糊不清。但是稍微的不齐不会影响跑胶结果。如果客户实在在意，可以按照两步法来做，先用水压平后再加上层胶。", "solution": "建议客户加上层胶的时候要缓慢，或者可以按照两步法来做，先用水压平后再加上层胶", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260511034"}, {"sales": "刘静文", "product": "BR1A401", "category": "快速PCR酶", "group": "李璇玑课题组", "date": "2026.5.21", "competitor": "诺唯赞p223", "direction": "负", "feedback": "客户快p测试没有条带", "detail": "客户试剂保存没有问题，在有效期内。客户没有做竞品对照，测试了几个样品没用条带。", "solution": "了解到客户用的之前的扩增程序，1000bp左右的条带扩延伸时间1min，且退火温度55°，建议客户按照说明书的程序时间和温度再试一下看看能不能有结果。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "待客户下次测试", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "杨柳课题组", "date": "2026.5.21", "competitor": "自己配制蛋白胶", "direction": "负", "feedback": "客户反馈上层胶无法把样品压成一条线", "detail": "客户反馈我们的浓缩胶呈现一条粗线无法压成一条线，与之前自己配制的蛋白胶做对比，自己配制的上层胶是浓缩胶可以压蛋白，但是一步法制胶的上层胶是没用浓缩功能的，已给客户解释。", "solution": "给客户解释了一步法制胶的上层胶原理，为了方便实验操作而专门设计的，这样的结构能确保在同一电压下完成电泳过程，减少因电压调整带来的操作麻烦，也能提升实验效率和结果稳定性。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1A503", "category": "直扩试剂", "group": "陶曾课题组", "date": "2026.5.19", "competitor": "", "direction": "负", "feedback": "客户测试，样本拟南芥扩增失败", "detail": "客户试剂保存没有问题，在有效期内。客户未做重复实验。客户样本是拟南芥，共测试了10个，仅有一个有微弱条带。并没有与竞品做对比，引物是经过验证的。", "solution": "线下了解后客户在模样的时候加了液氮，可能影响了裂解效果，给客户建议裂解液加150ul，直接研磨无需在加液氮。同时注意离心和延伸时间。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1F103", "category": "一步法qrt", "group": "金崇伟课题组", "date": "2026.5.18", "competitor": "", "direction": "负", "feedback": "qrt测试结果不好", "detail": "客户试剂保存没有问题，在有效期内。了解客户RNA质量，客户表示提取时就不太好，可能样本存在问题。", "solution": "客户后续重新提取样本后再测试，时间不确定。", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR1A503", "category": "直扩试剂", "group": "杨东风课题组", "date": "2026.5.15", "competitor": "", "direction": "负", "feedback": "丹参毛状根直扩扩增不出来。", "detail": "客户试剂保存没有问题，在有效期内。客户样品是浮萍叶片可以扩增，尝试毛状根效果不好，线下测试后减少了样本量，增加了裂解液的量，但是还是没用扩增，后续客户会提供样本和引物。", "solution": "后续根据客户提供样本和引物调整测试。", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR3N401", "category": "纯化磁珠", "group": "胡敏杰课题组", "date": "2026.5.13", "competitor": "", "direction": "负", "feedback": "联系人用贝克曼的磁珠多一个条带，用我们的还是没有解决。", "detail": "客户试剂保存没有问题，在有效期内。线下了解到客户纯化体系是35ul，之前用贝克曼的0.9×的无法去除230bp左右的条带，跟研发沟通后建议用我们的磁珠尝试1.2×，结果还是不理想，还是有残留。后客户不再尝试，后续继续跟进。", "solution": "", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1F103", "category": "一步法qrt", "group": "黄力全课题组", "date": "2026.5.12", "competitor": "", "direction": "负", "feedback": "客户反馈试用装是没问题的，但是上次买的那200t跑了两次出现问题，空的孔也有cq值，溶解曲线有双峰，但是样本和试用装用的一样", "detail": "具体信息客户最近较忙，没时间看。已和客户沟通，待客户有时间 线下沟通", "solution": "需线下跟进", "tech": "王凡", "is_product_issue": "否", "is_resolved": "线下跟进测试。做了以下条件优化：1.将RNA稀释至50ng/ul，20ul体系中加1ul。2.鉴于客户引物退火温度参差不齐，将退火问题从60提升至63，延伸时间从1min-30s。共测试了三个样本，10个基因。最后有七个基因正常。其余三个需要验证引物。基本排除产品问题。", "improvement": "客户测试后确定样本也存在问题", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR2B101", "category": "Trizol法提取试剂", "group": "张向臣课题组", "date": "2026.5.8", "competitor": "赛维尔-G3013", "direction": "负", "feedback": "客户用了trizol试剂发现离心之后还是混浊，且最后沉淀部分较少，浓度较低。", "detail": "客户试剂保存没有问题，在有效期内。线下了解到客户在加完氯仿混匀这一步混匀不完全，导致上清分离不充分。与竞品同时测试，竞品分离正常。客户共提取30个样本，其中用我们的测试了5个，可能存在混匀问题。", "solution": "线下实验时调整了加完氯仿后的涡旋时间，上清已经正常。但是有些发黄，可能是样本量太多有脂质残留。但不影响结果。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR1A503", "category": "直扩试剂", "group": "杨东风课题组", "date": "2026.5.8", "competitor": "", "direction": "负", "feedback": "客户反馈用直扩试剂扩增7个基因只有两个有条带。", "detail": "客户测试两次结果均不好，试剂存储和效期没有问题。样本是浮萍叶片，做转基因阳性鉴定。客户是用液氮研磨，样品去量较少且延伸时间设置较长。需进行优化", "solution": "线下测试做了一下优化。1.样品去量增加，共取四片叶子。2.裂解时手动捣碎，裂解液加量100ul。3.引物退火问题提高2度，时间为5s。4.客户使用10ul体系，模板上样量0.5ul。七个基因全部扩增成功。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260428001"}, {"sales": "王慧洁", "product": "BR1A503", "category": "直扩试剂", "group": "林福呈课题组", "date": "2026.5.6", "competitor": "", "direction": "负", "feedback": "客户做了48个菌类，只有四个成功扩增。", "detail": "客户试剂保存没有问题，在有效期内。之前线下帮客户做测试，共测试了两个菌种，可以扩增。但是这次客户实验是真菌分类，真菌种类较多，每个的形态都不一致，测试了48个样本只有4个成功。", "solution": "客户最近需要继续进行。且样本还需观察后续的菌落状态，所以不能提供样本。只有一个富裕的样本可以提供。已经拿分公司保存，待RNA样本收集完成后统一寄回测试。", "tech": "王凡", "is_product_issue": "是", "is_resolved": "待样本收集完成后寄回总部测试。丰富使用场景。", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR4D421", "category": "快速转膜液", "group": "吴海滨课题组", "date": "2026.4.28", "competitor": "", "direction": "负", "feedback": "客户反馈转膜液有一块胶转好了", "detail": "产品储存正常，产品在保质期内。客户用我们转膜液的同时转了两块胶，400mA，30min。蛋白大小是100kDa左右。之前是自己配制的转膜液", "solution": "1.鉴于客户同时转了两块胶但是其中一块有问题，排除产品问题。2.建议客户在转膜时，三明治结构要夹紧不能有偏移，且气泡要排干净。", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260311013"}, {"sales": "王慧洁", "product": "BR1A503", "category": "直扩试剂", "group": "苗惠莹课题组", "date": "2026.4.28", "competitor": "", "direction": "负", "feedback": "客户反馈直扩试剂没有扩出条带", "detail": "产品储存正常，产品在保质期内。客户模板是芥菜叶片，客户用的是手动裂解，其可能样品取量大且手动裂解不完全，导致没有扩增成功。没有与竞品做平行测试。", "solution": "1.线下推荐客户用研磨仪研磨，同时裂解液100-150ul。2.已约客户做线下测试", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR3M121", "category": "高保真酶", "group": "牟晓洲课题组", "date": "2026.4.24", "competitor": "", "direction": "负", "feedback": "客户反馈高保真扩增条带胡成一团", "detail": "产品储存正常，产品在保质期内。客户模板是cDNA，CDS序列扩增。客户引物是加同源臂的序列，客户按照退火70度来设置的程序，延伸时间按照5s/kb。没有和竞品做平行实验，但是客户表示其他样本用竞品没有问题。了解到，竞品扩增没出条带的，我们的酶是涂抹型条带", "solution": "1.了解到客户是按照加完同源臂后的退火来设置的，建议客户按照引物原退火设置。2.需看客户进一步测试结果", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "曾琪", "product": "BR4C351", "category": "蛋白Loading", "group": "王国珍课题组", "date": "2026.4.22", "competitor": "", "direction": "负", "feedback": "客户反馈loading不齐", "detail": "客户反馈loading不平，但是没有给具体的数据图片。", "solution": "如果跑电泳的时候蓝色条带是整齐的话就和loading的关系不大。最后显影不平的话，可能是转膜时候的问题。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "客户后续没有提供数据", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260417015"}, {"sales": "袁殿果", "product": "BR3M121", "category": "高保真酶", "group": "吴振芳课题组", "date": "2026.4.21", "competitor": "", "direction": "负", "feedback": "高保真测序多了一段序列", "detail": "高保真测序有一个多出58个引物上碱基，另一个多出253个引物上的碱基，引物长度30碱基", "solution": "推荐引物减半但客户没有再试，因为用takara的按照之前的体系测序没问题", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260317028"}, {"sales": "王慧洁", "product": "BR1F103", "category": "一步法qrt", "group": "徐华东课题组", "date": "2026.4.21", "competitor": "", "direction": "负", "feedback": "融解曲线有双峰导致CT值偏小，不稳定", "detail": "带教客户课题组做一步法qpcr实验，讲解加样及操作注意事项和曲线图分析。结果出来后，共测试四对引物，其中有两对有非特异性扩增，两对正常。客户后续再做测试，看引物是否存在问题。", "solution": "跟进客户后续引物测试情况", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260416015"}, {"sales": "袁殿果", "product": "BR1E102", "category": "一步法逆转录、qPCR酶", "group": "李林课题组", "date": "2026.4.17", "competitor": "", "direction": "负", "feedback": "客户试用了之后觉得我们与诺唯赞差距较大，后续也不考虑换产品，同一块板跑的数据，但不方便透露诺唯赞的数据", "detail": "只拿到我们的测试数据，从复孔重复性和数据上来看，没有问题。但是客户表示和诺唯赞差距较大，但是没有提供数据，也不同意线下了解。初步判断不是产品性能问题", "solution": "继续更客户沟通看是否同意进一步了解", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260317042"}, {"sales": "王慧洁", "product": "BR3M121", "category": "高保真酶", "group": "牟晓洲课题组", "date": "2026.4.17", "competitor": "", "direction": "负", "feedback": "高保真扩增不出来", "detail": "客户用我们的高保真扩增不出来，片段大小1500bp，时间是1min。用其他的不知名酶可以扩增出来", "solution": "建议客户按照说明书重新设置延伸时间，并可适当调整退火温度", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260205006"}, {"sales": "袁殿果", "product": "BR1E102", "category": "qPCR酶", "group": "方宏博课题组", "date": "2026.4.16", "competitor": "", "direction": "负", "feedback": "客户反馈组间内参CT值不齐", "detail": "客户反馈用我们的qpcr数据做出来组间内参CT值不齐，且有的组内复孔重复性不好，线下分析融解曲线和扩增曲线后发现，客户引物存在非特异性扩增，且RNA提取过程中醇和盐类残留较多，会影响扩增效率。已给客户建议，下周指导客户实验。", "solution": "已给客户建议，cDNA可稀释10倍，RNA提取过程中可多晾干一会，延伸时间可以设为30s。下周指导客户实验。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是，线下测试后无问题", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王惠洁", "product": "BR1D303", "category": "一步法逆转录", "group": "吕建新课题组", "date": "2026.4.15", "competitor": "", "direction": "负", "feedback": "逆转录数据异常，扩增曲线异常，无CT值", "detail": "一、样本信息\n\n1.客户样本共两种：（1）小鼠肝原代细胞（2）小鼠体内肝细胞提取后和OE细胞共培养\n\n2.RNA提取方法、浓度及质量：Trizol法提取。原代细胞RNA浓度200ng/ul左右， 共培养细胞100+ng/ul。 RNA质量：Nanodrop测定，260/230偏低，有一定有机溶剂残留", "solution": "已和之前问题归类反馈研发，跟进优化进度", "tech": "王凡", "is_product_issue": "是", "is_resolved": "否", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1D303 BR1E102", "category": "一步法逆转录、qpcr", "group": "王曦课题组", "date": "2026.4.10", "competitor": "", "direction": "负", "feedback": "客户逆转录qpcr后一点数据都没有", "detail": "客户反馈用我们的逆转录后跑qpcr结果一点数据都没有，后续qpcr用的翌圣的和我们的都没有数据", "solution": "客户没有提供数据，但是了解到客户是没有任何数据，大概率是程序或者操作问题，后续会线下了解", "tech": "王凡", "is_product_issue": "否", "is_resolved": "客户表示是没有CT值，线下找客户，客户急着去开组会，且数据在光盘里，后续会拷贝", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260331010"}, {"sales": "赵以诺", "product": "BR4D421", "category": "快速转膜液", "group": "杨巍课题组", "date": "2026.4.10", "competitor": "", "direction": "负", "feedback": "封闭后转膜花，没有内参", "detail": "客户反馈用我们的封闭液内参没有显影出来，剥离后用封闭奶粉后可以，目前客户还有提供具体数据图，初步判断客户抗体可能不适合快速封闭液，后续需要客户提供数据图和抗体信息", "solution": "初步判断客户抗体可能不适合快速封闭液，需要客户提供数据图和抗体信息，进一步分析", "tech": "王凡", "is_product_issue": "否", "is_resolved": "待了解客户信息", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1D303", "category": "一步法逆转录", "group": "蒋丽课题组", "date": "2026.4.8", "competitor": "", "direction": "负", "feedback": "客户反馈逆转录定量测试结果不行", "detail": "具体了解到客户RNA用AG的做效果也不好，判断客户RNA质量提取有问题，建议客户再次提取RNA", "solution": "建议客户重新提取RNA，并且跑胶验证后再做后续实验", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR1A401", "category": "快速PCR酶", "group": "李佳课题组", "date": "2026.4.8", "competitor": "", "direction": "负", "feedback": "客户扩增2kb，片段出现非特异性扩增", "detail": "客户做基因分型鉴定，用我们的快速PCR扩增，可以扩增出来目的片段，但是有一条非特异性的条带，了解到客户引物退火是用的之前的，且延伸时间设置较长，可能会出现非特异性扩增", "solution": "建议客户提高2-3度退火温度，且延伸时间设置在5s内", "tech": "王凡", "is_product_issue": "否", "is_resolved": "待测试结果", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260317047"}, {"sales": "袁殿果", "product": "BR3M121", "category": "高保真酶", "group": "吴振芳课题组", "date": "2026.4.8", "competitor": "", "direction": "负", "feedback": "高保真扩增多出引物片段", "detail": "客户用高保真酶扩增质粒，20个循环，扩增结束后测序发现多了一段引物的序列，其余都正常。判断客户引物加量多，在扩增质粒等简单长片段时会出现多扩增引物的现象。", "solution": "建议客户引物量减半再测试", "tech": "王凡", "is_product_issue": "否", "is_resolved": "待测试结果", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1D301 BR1E102", "category": "两步法逆转录、qpcr", "group": "夏到宗课题组", "date": "2026.4.8", "competitor": "", "direction": "负", "feedback": "有几个孔没有CT值", "detail": "客户做的细胞RNA的逆转录和qpcr，客户反馈有三孔没有CT值，拿到客户数据分析后发现，三个孔都是三个不同的技术重复中的，另外来两个是没有问题的。", "solution": "结合三完全相同的复孔中有一个没有数据，判断客户可能加样发生漏加。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "袁殿果", "product": "BR4D421", "category": "快速转膜液", "group": "黄云鹏课题组", "date": "2026.4.3", "competitor": "", "direction": "负", "feedback": "客户转膜液，效果不是很理想，大于70的蛋白30分钟转不了，40分钟能转，条带看着也怪怪的。", "detail": "客户的内参蛋白条带跑的大小不一，且浓度也不一致，结合和的蛋白样品是两周之前的，推测客户的样品可能出现讲解", "solution": "建议客户摸索转膜条件，更换样品后重新测试", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1D301 BR1E102", "category": "两步法逆转录、qpcr", "group": "任孝军课题组", "date": "2026.4.2", "competitor": "", "direction": "负", "feedback": "客户反馈逆转录定量测试结果不如诺唯赞效果不行", "detail": "客户目前要更换逆转录试剂，客户不做gDNA去除操作，样本是细胞RNA。分别测试了诺唯赞，近岸和我们的两步法逆转录，货号如图。相同的内参基因，诺唯赞CT值小，近岸和我们的相差不大。相同的目的靶标，我们的CT和诺唯赞相差不大，近岸有多个无CT。所以整体趋势诺唯赞的符合预期，我们的相反", "solution": "已将问题反馈研发", "tech": "王凡", "is_product_issue": "是", "is_resolved": "否", "improvement": "研发在搭建体系测试", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1D301 BR1E102", "category": "两步法逆转录、qpcr", "group": "邱石桥课题组", "date": "2026.4.2", "competitor": "", "direction": "负", "feedback": "客户反馈逆转录定量测试结果不行", "detail": "客户暂时没有提供数据", "solution": "后续线下了解具体情况", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1D301 BR1E102", "category": "两步法逆转录、qpcr", "group": "夏到宗课题组", "date": "2026.3.31", "competitor": "", "direction": "负", "feedback": "做了两次，第一次一步法，平行组重复性不对，第二次两步法，趋势不对", "detail": "数据分析后，客户的内参CT值没有问题且三个复孔也没有问题，客户反馈趋势不符WB趋势，推测有可能蛋白出现翻译后修饰导致的。", "solution": "建议客户统一批次验证，及查阅文献看蛋白修饰结果", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "曾琪", "product": "BR3M121", "category": "高保真酶", "group": "童汉华课题组", "date": "2026.3.30", "competitor": "", "direction": "负", "feedback": "客户反映用快p酶和高保真酶拿来扩用来扩2000bp以下的短片段还是可以的，但是上了2000就没那么好了。三个两千多三千多bp的片段，都没成功", "detail": "客户同时测试了Takara，赛默飞和宝锐的高保真酶，了解到客户扩增片段是3000bp，GC含量70+，只有赛默飞的扩增成功。客户用的延伸时间是1min，退火温度没有摸索。", "solution": "建议客户按照我们说明书来设置程序，同时可以摸索退火温度", "tech": "王凡", "is_product_issue": "否", "is_resolved": "待客户二次测试", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260316022"}, {"sales": "袁殿果", "product": "BP-17-05", "category": "CircRNA 合成试剂盒一步法", "group": "余露山课题组", "date": "2026.3.30", "competitor": "", "direction": "负", "feedback": "客户反馈一步法的试剂盒效果比较差，产物太少了，效率有点低", "detail": "一步法CircRNA 合成效率原理上是低于正常的两步法的，已反馈研发", "solution": "已反馈mRNA研发", "tech": "王凡", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260319002"}, {"sales": "赵以诺", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "夏大静课题组", "date": "2026.3.30", "competitor": "", "direction": "负", "feedback": "客户反馈上下层胶分界线不清楚", "detail": "客户反馈上下层胶分界线不平，推测客户加上层胶时太快导致，上层胶过度冲刷下层胶，导致分界线不清楚，该现象不影响正常使用。", "solution": "建议客户加上层胶的时候要缓慢，可以来回滑动加。如果客户实在避免不了，可以采用两步法来做，先液封再加上层胶", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260318028"}, {"sales": "王慧洁", "product": "BR1A503", "category": "直扩试剂", "group": "陈昆松课题组", "date": "2026.3.30", "competitor": "", "direction": "负", "feedback": "草莓和番茄叶片测试没有扩增条带，其中一个有涂抹性条带", "detail": "了解到客户测试时是手动研磨捣碎的，且扩增体系用的是10ul体系，模板量加样是1ul，结合番茄和草莓多糖多酚的特点，推测可能是客户研磨不充分加之模板加量多导致抑制PCR反应。", "solution": "推荐客户用机器研磨，加长裂解时间，同时减少模板加量再测试。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "否，待客户测试", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1A503", "category": "直扩试剂", "group": "苏珍珠课题组", "date": "2026.3.26", "competitor": "", "direction": "负", "feedback": "直扩试剂测试出现双峰", "detail": "客户实验是分土壤菌类型，需要涉及大量不同种属的菌，测试直扩试剂扩增出现双峰，后续线下帮客户测试", "solution": "线下帮客户测试，但由于菌的种类太多，不确定是否可以适用", "tech": "王凡", "is_product_issue": "否", "is_resolved": "等二次测试结果", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260317005"}, {"sales": "赵以诺", "product": "BR4D441", "category": "快速转膜液", "group": "蔡志坚课题组", "date": "2026.3.24", "competitor": "", "direction": "负", "feedback": "客户测试快速转膜液反馈大分子条带转不干净", "detail": "客户测试快速转膜液反馈大分子条带转不干净，条件是400mA,30min，150kDa的条带转膜不干净，Maker是用的雅酶的。", "solution": "询问相应研发工程师后确定，，按照说明书条件转我们的maker 是没有问题的，但是其他品牌的可能会出现这样的情况，需要进一步摸索条件", "tech": "王凡", "is_product_issue": "否", "is_resolved": "等客户后续测试结果", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260317023"}, {"sales": "王慧洁", "product": "BR1D303", "category": "一步法逆转录", "group": "陈栩燊", "date": "2026.3.20", "competitor": "", "direction": "负", "feedback": "客户逆转录后qpcr曲线数值异常", "detail": "根据客户给的数据及加样分析，客户RNA模板加样500ng，后qpcr体系上样1.5ul。结合CT值小的结果，所以建议客户逆转录RNA量减少，或者模板可以稀释100倍。后客户用诺唯赞的结果可以做出来，线下了解后发现，客户模板稀释5倍，诺唯赞也有两个样本数据异常", "solution": "结合陈昆松组问题，同样是部分样本异常，加样量多", "tech": "王凡", "is_product_issue": "是", "is_resolved": "否", "improvement": "", "progress": "研发结合陈昆松课题组物料测试，正在做更多情况的测试，排查问题", "follow_up": "是", "order_no": "ZHBR260123010"}, {"sales": "王慧洁", "product": "BR1D303", "category": "一步法逆转录", "group": "庄汝杰课题组", "date": "2026.3.17", "competitor": "", "direction": "负", "feedback": "逆转录后CT值特别小", "detail": "客户用我们的逆转录和诺唯赞的逆转录跑qpcr有一些样本跑的不好，还有几个孔没跑出来，经过扩增曲线和融解曲线分析，发现客户有一个靶标引物试剂可能有问题，建议重新设计，同时客户CT值整体偏小，建议客户稀释模板后再测试。", "solution": "建议客户稀释模板后再测试，同时可以重新设计引物", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1A503", "category": "直扩试剂", "group": "斯金平课题组", "date": "2026.3.12", "competitor": "", "direction": "负", "feedback": "客户反馈直扩试剂测试没有条带", "detail": "客户用直扩反馈不好，跟进了一下操作细节，裂解15min，加了1μl模板，然后加了100μL的Plant Lysis Buffer。", "solution": "线下帮客户测试，裂解时间20min，模板加样2ul，可以扩增出条带，但是有非特异性条带，建议提高退火温度", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260206073"}, {"sales": "王慧洁", "product": "BR1D303", "category": "一步法逆转录", "group": "陈昆松课题组", "date": "2026.3.11", "competitor": "", "direction": "负", "feedback": "逆转录有一部分样本逆转录不出来导致CT值异常", "detail": "客户使用同一管BR1D303共做了三次实验，逆转录后qpcr试剂为翌圣11184ES。第一次实验没有问题。第二次实验有个别孔出现问题，客户以为是加样错误没有在意。（前两次都在年前）.第三次实验有一半的样品没有CT值。同时让客户用无CT值的产物跑胶，是有条带的。后客户用相同的RNA和诺唯赞两步法逆转录", "solution": "已经将客户物料寄回研发测试", "tech": "王凡", "is_product_issue": "是", "is_resolved": "否", "improvement": "", "progress": "客户提供的cDNA确实可以复现客户结果，测试研发自备RNA样本，没有问题，进一步探究中", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1F101", "category": "一步法定量", "group": "张大宏课题组", "date": "2026.2.4", "competitor": "", "direction": "负", "feedback": "测试CT值偏大26左右", "detail": "qrt的测试效果不行，ct值很高，大概在28，29左右，怀疑是引物问题。建议客户重新设计引物，产物要短一些更好", "solution": "客户后续设计引物测试", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR260126001"}, {"sales": "王慧洁", "product": "BR3M121", "category": "高保真酶", "group": "牟晓洲课题组", "date": "2026.2.3", "competitor": "", "direction": "负", "feedback": "高保真酶扩增有点突变", "detail": "客户用我们高保真酶构建点突变。一共需要扩增20多个ta，长度大概是7k左右。之前构建的载体转化后挑单克隆有的测序没问题，有的会有插入缺失。但是客户最近构建的一个载体有一个位点发生了点突变，且挑了四个单克隆都是同样的位置，大概率是扩增中发生了突变，已建议客户再扩增一下。单个碱基突变会有一定的概率。", "solution": "客户扩增多个出现一个点突变，建议客户多扩增一次，属正常现象", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR4C602", "category": "ECL显影液", "group": "杨彩梅课题组", "date": "2026.1.29", "competitor": "", "direction": "负", "feedback": "客户反馈ECL显影慢，暴光时间长", "detail": "了解到客户用的是fg级的ECL显影液，且客户条带浓度底，所以我们的pg级的显影时间会慢，已跟客户表示，后续有fg级向客户推荐", "solution": "是", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1D303", "category": "一步法逆转录", "group": "茹磊课题组", "date": "2026.1.28", "competitor": "", "direction": "负", "feedback": "客户逆转后qpcr结果有问题", "detail": "客户测试逆转录和定量，用我们的逆转录反转的cDNA跑的产物量很低，导致有的没有CT值，其他的逆转录出来的就没有问题，其他条件都一样。现在等客户反馈程序设置和模板是否高GC，后续再排查问题。", "solution": "线下测试后确实逆转录 条带较暗，同时客户逆转录模板加样量多，有可能影响", "tech": "王凡", "is_product_issue": "是", "is_resolved": "否", "improvement": "", "progress": "同批次样品测试，结果没有问题，后续更多测试结果在分析中", "follow_up": "是", "order_no": ""}, {"sales": "李岚风", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "谭峰课题组", "date": "2026.1.23", "competitor": "", "direction": "负", "feedback": "客户反馈下层胶凝的快，上层胶慢", "detail": "下层胶凝太快，上层胶凝太慢，浓缩胶加下去和下层胶的界面就会歪七扭八的，了解到客户促凝剂加样问题", "solution": "建议客户下层胶少加促凝剂，同时上层胶加样的时候要缓慢，分界线不求也不影响结果", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR251229036"}, {"sales": "李岚风", "product": "BR1A401", "category": "快速PCR酶", "group": "王艳丽课题组", "date": "2026.1.22", "competitor": "", "direction": "负", "feedback": "客户反馈快速pcr没有扩增出来", "detail": "了解到客户还是用的以前的程序而且，退火温度没有摸索所以导致没有条带。", "solution": "建议客户按照说明书的程序来，同时找到最适退火温度，客再次测试问题已经解决。", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "是", "order_no": ""}, {"sales": "王慧洁", "product": "BR1D303", "category": "一步法逆转录", "group": "任雪峰课题组", "date": "2026.1.22", "competitor": "", "direction": "负", "feedback": "客户反馈用我们的没有做出来", "detail": "客户是新手，做的时候用我们的没有做出来用AB克隆的没有问题，了解到可能是客户操作问题。建议客户再次测试", "solution": "线下去客户端操作测试没有问题", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "是", "order_no": "ZHBR251203016"}, {"sales": "王慧洁", "product": "BR1D303", "category": "一步法逆转录", "group": "陈昆松课题组", "date": "2026.1.21", "competitor": "", "direction": "负", "feedback": "逆转录后qpcr出现问题", "detail": "客户反馈逆转录后跑qpcr出现问题，但是之前做的最近才反馈，数据和样本都没有保留，等客户后续再测试。", "solution": "客户没有保留任何数据所以，等客户后续再次测试", "tech": "", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "是", "order_no": "ZHBR251116003"}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "梁广课题组", "date": "2026.1.20", "competitor": "", "direction": "负", "feedback": "一步法胶测试效果不好", "detail": "客户用的10%的胶，感觉上下层分界线不明显，客户觉得跑出来效果不好", "solution": "已跟客户说明有优化版本的，可以再次测试", "tech": "", "is_product_issue": "是", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "是", "order_no": "ZHBR251211015"}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "夏到宗课题组", "date": "2026.1.8", "competitor": "", "direction": "负", "feedback": "客户反馈测试我们蛋白胶有两个孔跑歪了", "detail": "线下拜访客户，从图上来看，很可能是转膜的滤纸有褶皱或者破了，因为了解到客户在电泳时没有异常", "solution": "建议客户更换滤纸，或者待组内他人再次测试", "tech": "", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "罗福全课题组", "date": "2026.1.6", "competitor": "", "direction": "负", "feedback": "客户测蛋白胶跑出哑铃型条带", "detail": "客户测试蛋白胶跑出来了哑铃装条带，有两种情况可能导致，1胶凝的不充分，2.蛋白样品有杂质，或者加loding buffer后没有混匀，导致不充分。", "solution": "客户蛋白maker忘记了条带是否是哑铃状，但是客户说换了胶就没问题。建议客户凝胶时间充足一些，加样混匀在测试。", "tech": "", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "杨珺课题组", "date": "2025.12.30", "competitor": "", "direction": "负", "feedback": "客户反馈上下层胶分界稍有不平", "detail": "根据课题提供的胶图来看，分解线有一点不平，客户反馈说不敢用", "solution": "已告知客户加上层胶的时候缓慢，而且不影响使用", "tech": "", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1A401", "category": "快速PCR酶", "group": "刘梦柱课题组", "date": "2025.12.26", "competitor": "", "direction": "负", "feedback": "客户测试快速PCR，条带是涂抹的", "detail": "根据客户提供的胶图来看，大概率是模板降解了导致没有主带，都是涂抹装的，已建议客户更换模板再测试", "solution": "客户更换模板后没有问题了", "tech": "王凡", "is_product_issue": "否", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": "ZHBR251205014"}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "李昌煜课题组", "date": "2025.12.25", "competitor": "", "direction": "负", "feedback": "客户反馈凝胶时间久", "detail": "客户上层胶加下层胶凝胶用了一个小时，觉得时间太久", "solution": "研发已推出优化后的制胶试剂盒", "tech": "王凡", "is_product_issue": "是", "is_resolved": "是", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "黄建佳", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "", "date": "2025.12.24", "competitor": "", "direction": "负", "feedback": "用的一步法配胶试剂盒反馈了条带连在一起", "detail": "客户采用的先低压后高压的电泳跑的，时间较长可能温度升高，导致条带有异常，建议客户150v 1h", "solution": "客户测试后效果还是不好，内参不齐，初步判断是转膜问题，建议客户转膜时气泡赶赶紧，滤纸，膜和胶之间要压紧", "tech": "王凡", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "余洁茹课题组", "date": "2025.12.19", "competitor": "", "direction": "负", "feedback": "客户试用蛋白胶20min还没有凝胶", "detail": "客户测试一步法制胶，凝胶20min看胶还没凝，以为有问题，就没有继续做", "solution": "客户室温比较低，已经告知客户多加促凝剂。客户后续会再测试一次。", "tech": "", "is_product_issue": "是", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "王楠课题组", "date": "2025.12.18", "competitor": "", "direction": "负", "feedback": "客户测试一步法制胶，感觉跑出来条带有些毛刺", "detail": "客户试用我们的一步法制胶，感觉跑的蛋白Maker条带有点毛刺，但是从客户提供的图片来看，看不出来明显区别，曝光后条带没用影响。", "solution": "客户用的是180V电压跑的，电压太高，且电泳液回收次数过多，会导致条带不好看的现象，建议客户用150V，新电泳液跑胶。", "tech": "", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1F101", "category": "一步法定量", "group": "郭勇课题组", "date": "2025.12.17", "competitor": "", "direction": "负", "feedback": "一步法定量客户预实验没有做出来", "detail": "客户之前测试qrt试剂，做的预实验，实验结果不理想，分析后可能是客户引物设计和实验不合理，后来客户发现确实存在错误", "solution": "建议客户重新设计引物和实验", "tech": "", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1F101", "category": "一步法定量", "group": "罗自生课题组", "date": "2025.12.17", "competitor": "", "direction": "负", "feedback": "客户内参没有问题，但是目前基因CT值很靠后", "detail": "客户测试qrt，一共跑了两个基因，一个是内参，另一个是目的基因。结果是内参的CT值正常，但是目的基因CT值偏大，大概是在30左右，融解曲线没有问题", "solution": "经了解客户目的基因在果实中表达量较高，这次测试的是叶片中的表达量，可能本身表达量就不太高。后续会再做测试。", "tech": "否", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "黄自鑫", "product": "BR4C333", "category": "一步法制胶试剂盒", "group": "张敏课题组", "date": "2025.12.16", "competitor": "", "direction": "负", "feedback": "客户觉得一步法制胶瓶中溶液气泡多", "detail": "客户用了我们的配胶试剂盒，反馈有个问题就是打开后气泡很多，影响她配比", "solution": "建议客户从4度拿出来后在室温平衡几分钟气泡会少很多", "tech": "否", "is_product_issue": "是", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR1A503", "category": "直扩试剂", "group": "黄家保课题组", "date": "2025.12.16", "competitor": "", "direction": "负", "feedback": "我们的直扩野生型能做出来，突变型做不出来，之前试过很多家的直扩都做不出来，老师让我们那突变型的回去做一下定制", "detail": "寄回研发测试后，发现突变体的需要裂解20-25分钟效果更好，同时客户要求多配些裂解液用机器研磨", "solution": "已重新给客户发测试，后续帮客户测试", "tech": "", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "王慧洁", "product": "BR3M121", "category": "高保真酶", "group": "林福呈课题组", "date": "2025.12.15", "competitor": "", "direction": "负", "feedback": "客户用我们的高保真酶杂带多", "detail": "客户课题组已经采购高保真酶，公用的同时还有其他酶，客户反馈我们的酶扩增有杂带，线下了解到客户目的片段大概是3k左右但是延伸时间是1min，时间太长导致出现非特异性扩增。", "solution": "建议客户按照说明推荐延伸时间来做", "tech": "", "is_product_issue": "否", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}, {"sales": "刘静文", "product": "BR1A401", "category": "快速PCR酶", "group": "桂任意课题组", "date": "2025.12.12", "competitor": "", "direction": "负", "feedback": "客户测试快速pcr没有条带", "detail": "客户测试我们快速pcr，目前片段是3000bp，但是客户设置的延伸时间是8s，我们的可以做到2kb以下每秒每kb", "solution": "2kb以上建议客户按照5s每kb来，建议客户设置15-20s，延伸时间。", "tech": "", "is_product_issue": "", "is_resolved": "", "improvement": "", "progress": "", "follow_up": "", "order_no": ""}], "period": "2025-12 ~ 2026-07"}